ARG57143
anti-PSME1 antibody [12H3]
anti-PSME1 antibody [12H3] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [12H3] recognizes PSME1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 12H3 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | PSME1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-249 of Human PSME1 |
| 偶联标记 | Un-conjugated |
| 別名 | REG-alpha; Proteasome activator 28 subunit alpha; PA28alpha; Activator of multicatalytic protease subunit 1; 11S regulator complex subunit alpha; Interferon gamma up-regulated I-5111 protein; PA28A; IGUP I-5111; IFI5111; Proteasome activator complex subunit 1; REGalpha; PA28a |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q06323 Human Proteasome activator complex subunit 1 |
|---|---|
| 基因名称 | PSME1 |
| 全名 | proteasome activator subunit 1 |
| 背景介绍 | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 生物功能 | Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. [UniProt] |
| 预测分子量 | 29 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG57143 anti-PSME1 antibody [12H3] WB image
Western blot: 40 µg of 1) HeLa, 2) LNCap, 3) A549, 4) 293T, 5) Jurkat, 6) Raji, and 7) A431 cell lysates stained with ARG57143 anti-PSME1 antibody [12H3] at 1:1000.
