ARG57083
anti-PSMD11 antibody [2C7]
anti-PSMD11 antibody [2C7] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [2C7] recognizes PSMD11 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 2C7 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | PSMD11 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-422 of Human PSMD11. |
| 偶联标记 | Un-conjugated |
| 別名 | S9; 26S proteasome non-ATPase regulatory subunit 11; Rpn6; p44.5; 26S proteasome regulatory subunit S9; 26S proteasome regulatory subunit RPN6; 26S proteasome regulatory subunit p44.5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00231 Human 26S proteasome non-ATPase regulatory subunit 11 |
|---|---|
| 基因名称 | PSMD11 |
| 全名 | proteasome 26S subunit, non-ATPase 11 |
| 背景介绍 | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012] |
| 生物功能 | Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity. [UniProt] |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Phosphorylated by AMPK. |
检测图片 (1) Click the Picture to Zoom In
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ARG57083 anti-PSMD11 antibody [2C7] WB image
Western blot: 40 µg of 1) HeLa cell lysate, 2) 293T cell lysate, 3) U87-MG cell lysate, 4) NIH-3T3 cell lysate, 5) PC3 cell lysate, 6) TF1 cell lysate, 7) MCF7 cell lysate stained with ARG57083 anti-PSMD11 antibody [2C7] at 1:1000.
