ARG43994
anti-PSMC4 antibody
anti-PSMC4 antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PSMC4 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PSMC4 |
| 抗原物种 | Human |
| 抗原 | Human PSMC4 recombinant protein |
| 表达系统 | E.coli |
| 偶联标记 | Un-conjugated |
| Protein Full name | 26S proteasome regulatory subunit 6B |
| 別名 | PSMC4; Proteasome 26S Subunit, ATPase 4; TBP-7; TBP7; Tat-Binding Protein 7; MIP224; RPT3; S6; Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 4; 26S Proteasome AAA-ATPase Subunit RPT3; 26S Proteasome Regulatory Subunit 6B; MB67-Interacting Protein; Protease 26S Subunit 6; MGC13687; MGC23214; MGC8570; 26S Protease Regulatory Subunit 6B; Proteasome 26S Subunit ATPase 4; MB67 Interacting Protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified with Immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PSMC4 |
| 全名 | Proteasome 26S Subunit, ATPase 4 |
| 背景介绍 | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| 生物功能 | Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC4 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides. |
| 预测分子量 | 47 kDa |
| 翻译后修饰 | Acetylation, Phosphoprotein |
检测图片 (7) Click the Picture to Zoom In
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ARG43994 anti-PSMC4 antibody ICC/IF image
Immunofluorescence: U20S stained with ARG43994 anti-PSMC4 antibody.
From Rummens J et al. Neuron. (2025), doi: 10.1016/j.neuron.2025.03.004, Fig. S6. B.
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ARG43994 anti-PSMC4 antibody IHC-P image
Immunohistochemistry: Human adenocarcinoma stained with ARG43994 anti-PSMC4 antibody at 2 μg/ml dilution.
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ARG43994 anti-PSMC4 antibody WB image
Western blot: HepG2 and U251 stained with ARG43994 anti-PSMC4 antibody at 0.5 μg/mL dilution.
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ARG43994 anti-PSMC4 antibody FACS image
Flow Cytometry: U20S cells stained with ARG43994 anti-PSMC4 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43994 anti-PSMC4 antibody IHC-P image
Immunohistochemistry: Human glioblastoma stained with ARG43994 anti-PSMC4 antibody at 2 μg/ml dilution.
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ARG43994 anti-PSMC4 antibody WB image
Western blot: Rat liver and Rat brain stained with ARG43994 anti-PSMC4 antibody at 0.5 μg/mL dilution.
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ARG43994 anti-PSMC4 antibody WB image
Western blot: Mouse liver and Mouse brain stained with ARG43994 anti-PSMC4 antibody at 0.5 μg/mL dilution.
文献引用
TDP-43 seeding induces cytoplasmic aggregation heterogeneity and nuclear loss of function of TDP-43
ICC/IF / Human
