ARG66459
anti-PSMC3 / TBP1 antibody
anti-PSMC3 / TBP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PSMC3 / TBP1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Zfsh |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PSMC3 / TBP1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide within the center region of Human PSMC3 / TBP1. |
| 偶联标记 | Un-conjugated |
| 別名 | Proteasome 26S subunit ATPase 3; Proteasome subunit P50; TBP1; TBP-1; 26S protease regulatory subunit 6A; Tat-binding protein 1; 26S proteasome AAA-ATPase subunit RPT5 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 0.2% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17980 Human 26S protease regulatory subunit 6A |
|---|---|
| 基因名称 | PSMC3 |
| 全名 | proteasome 26S subunit, ATPase 3 |
| 背景介绍 | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008] |
| 生物功能 | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Colocalizes with TRIM5 in the cytoplasmic bodies. [UniProt] |
| 预测分子量 | 49 kDa |
| 翻译后修饰 | Sumoylated by UBE2I in response to MEKK1-mediated stimuli. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG66459 anti-PSMC3 / TBP1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human breast cancer. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then incubated with ARG66459 anti-PSMC3 / TBP1 antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG66459 anti-PSMC3 / TBP1 antibody WB image
Western blot: HeLa, HepG2 and HT29 whole cell lysates stained with ARG66459 anti-PSMC3 / TBP1 antibody.
