ARG59906
anti-PSMC3 / TBP1 antibody
anti-PSMC3 / TBP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PSMC3 / TBP1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PSMC3 / TBP1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-180 of Human PSMC3 (NP_002799.3). |
| 偶联标记 | Un-conjugated |
| 別名 | Proteasome 26S subunit ATPase 3; Proteasome subunit P50; TBP1; TBP-1; 26S protease regulatory subunit 6A; Tat-binding protein 1; 26S proteasome AAA-ATPase subunit RPT5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse testis | ||||||
| 实际分子量 | 49 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PSMC3 |
| 全名 | proteasome 26S subunit, ATPase 3 |
| 背景介绍 | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq, Jul 2008] |
| 生物功能 | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity). In case of HIV-1 infection, suppresses Tat-mediated transactivation. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Colocalizes with TRIM5 in the cytoplasmic bodies. [UniProt] |
| 预测分子量 | 49 kDa |
| 翻译后修饰 | Sumoylated by UBE2I in response to MEKK1-mediated stimuli. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG59906 anti-PSMC3 / TBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver stained with ARG59906 anti-PSMC3 / TBP1 antibody at 1:100 dilution.
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ARG59906 anti-PSMC3 / TBP1 antibody WB image
Western blot: 25 µg of Mouse testis lysate stained with ARG59906 anti-PSMC3 / TBP1 antibody at 1:1000 dilution.
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ARG59906 anti-PSMC3 / TBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney stained with ARG59906 anti-PSMC3 / TBP1 antibody at 1:100 dilution.
