ARG41010
anti-PSMB5 antibody
anti-PSMB5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PSMB5 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PSMB5 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-263 of Human PSMB5 (NP_002788.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Proteasome chain 6; Proteasome subunit beta type-5; Proteasome epsilon chain; LMPX; Proteasome subunit MB1; Macropain epsilon chain; EC 3.4.25.1; Multicatalytic endopeptidase complex epsilon chain; Proteasome subunit X; X; MB1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | BT474 | ||||||||||
| 实际分子量 | 21 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PSMB5 |
| 全名 | proteasome subunit beta 5 |
| 背景介绍 | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| 生物功能 | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. [UniProt] |
| 预测分子量 | 28 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG41010 anti-PSMB5 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG41010 anti-PSMB5 antibody.
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ARG41010 anti-PSMB5 antibody WB image
Western blot: 25 µg of BT474 cell lysate stained with ARG41010 anti-PSMB5 antibody at 1:1000 dilution.
