ARG43991

anti-PSMB2 antibody

anti-PSMB2 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes PSMB2
反应物种 Hu
应用 ELISA, FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PSMB2
抗原物种 Human
抗原 Human PSMB2 recombinant protein
表达系统 E.coli
偶联标记 Un-conjugated
Protein Full name Proteasome subunit beta type-2
別名 PSMB2; Macropain subunit C7-I; Proteasome subunit beta type-2; Multicatalytic endopeptidase complex subunit C7-I; HC7-I; Proteasome component C7-I; EC 3.4.25.1

应用说明

应用建议
应用 推荐稀释比
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified with Immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 5690 Human PSMB2

Swiss-port # P49721 Human Proteasome subunit beta type-2

基因名称 PSMB2
全名 Proteasome 20S Subunit Beta 2
背景介绍 The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
生物功能 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity. [UniProt]
细胞定位 Cytoplasm, Nucleus, Proteasome
预测分子量 23 kDa
翻译后修饰 Acetylation

检测图片 (3) Click the Picture to Zoom In

  • ARG43991 anti-PSMB2 antibody ICC/IF image

    Immunofluorescence:U2OS cells stained with ARG43991 anti-PSMB2 antibody at 5 μg/ml dilution.

  • ARG43991 anti-PSMB2 antibody WB image

    Western blot: 293T and HeLa stained with ARG43991 anti-PSMB2 antibody at 0.5 μg/mL dilution.

  • ARG43991 anti-PSMB2 antibody FACS image

    Flow Cytometry: Raji cells stained with ARG43991 anti-PSMB2 antibody (blue) at 1 μg/1x10^6 cells dilution.