ARG41022
anti-PSMB10 antibody
anti-PSMB10 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
参考文献1
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PSMB10 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PSMB10 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-273 of Human PSMB10 (NP_002792.1). |
| 偶联标记 | Un-conjugated |
| 別名 | LMP10; Proteasome MECl-1; Proteasome subunit beta-2i; Low molecular mass protein 10; Macropain subunit MECl-1; EC 3.4.25.1; Proteasome subunit beta type-10; MECL1; Multicatalytic endopeptidase complex subunit MECl-1; beta2i |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | THP-1 | ||||||||
| 实际分子量 | 29 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PSMB10 |
| 全名 | proteasome subunit beta 10 |
| 背景介绍 | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008] |
| 生物功能 | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. [UniProt] |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG41022 anti-PSMB10 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG41022 anti-PSMB10 antibody.
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ARG41022 anti-PSMB10 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG41022 anti-PSMB10 antibody at 1:200 dilution.
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ARG41022 anti-PSMB10 antibody WB image
Western blot: 25 µg of THP-1 cell lysate stained with ARG41022 anti-PSMB10 antibody at 1:5000 dilution.
文献引用
Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms
WB / Mouse
