ARG20545
anti-PSD95 antibody [6G6]
anti-PSD95 antibody [6G6] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Bovine,Human,Mouse,Rat
Neuroscience antibody
概述
| 产品描述 | Mouse Monoclonal antibody [6G6] recognizes PSD95 |
|---|---|
| 反应物种 | Hu, Ms, Rat, Bov |
| 应用 | ICC/IF, IHC-P, WB |
| 特异性 | Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6G6 |
| 同位型 | IgG2a |
| 靶点名称 | PSD95 |
| 抗原物种 | Rat |
| 抗原 | Recombinant Rat PSD-95 (NP_062567.1) |
| 偶联标记 | Un-conjugated |
| 別名 | Postsynaptic density protein 95; SAP90; PSD-95; Synapse-associated protein 90; PSD95; SAP-90; Disks large homolog 4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | Dlg4 |
| 全名 | discs, large homolog 4 (Drosophila) |
| 背景介绍 | Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex. These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins. Thus different PDZ domains of PSD-95 might be specialized for distinct functions. PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism. |
| 生物功能 | Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. [UniProt] |
| 细胞定位 | Cell Membrane, Cell Junction, Synapse |
| 产品亮点 | Related products: PSD95 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Neuronal Development Marker Viral-like capsids, new trans-synaptic mRNA transport mechanism |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 80 kDa |
| 翻译后修饰 | Palmitoylation of isoform 1 is required for targeting to postsynaptic density. |
检测图片 (3) Click the Picture to Zoom In
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ARG20545 anti-PSD95 antibody [6G6] ICC/IF image
Immunocytochemistry: Rat dissociated hippocampal neurons fixed by cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M Sodium phosphate buffer. Sample was stained with ARG20545 anti-PSD95 antibody [6G6] at 1:1000 dilution for 12 hours at 4°C.
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ARG20545 anti-PSD95 antibody [6G6] ICC/IF image
Immunofluorescence: HaCaT cells. Fixation: Cold 100% methanol for 10 min at -20°C. Primary Antibody: ARG20545 anti-PSD95 antibody [6G6] at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat anti-Mouse (green) at 1:50 for 1 hour at RT.
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ARG20545 anti-PSD95 antibody [6G6] WB image
Western blot: Rat membrane stained with ARG20545 anti-PSD95 antibody [6G6] at 1:1000 dilution.
