概述

产品描述	Rabbit Polyclonal antibody recognizes PRMT1
反应物种	Hu, Ms, Rat
应用	ChIP, ICC/IF, IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	PRMT1
抗原物种	Human
抗原	Synthetic peptide of Human PRMT1.
偶联标记	Un-conjugated
別名	IR1B4; EC 2.1.1.125; ANM1; Protein arginine N-methyltransferase 1; Histone-arginine N-methyltransferase PRMT1; HCP1; EC 2.1.1.-; Interferon receptor 1-bound protein 4; HRMT1L2

应用说明

应用建议	应用 推荐稀释比 ChIP Assay-dependent ICC/IF 1:100 - 1:500 IHC-P 1:50 - 1:200 WB 1:500 - 1:1000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照	NIH/3T3
实际分子量	~ 42 kDa

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 15469 Mouse PRMT1 GeneID: 3276 Human PRMT1 GeneID: 60421 Rat PRMT1 Swiss-port # Q63009 Rat Protein arginine N-methyltransferase 1 Swiss-port # Q99873 Human Protein arginine N-methyltransferase 1 Swiss-port # Q9JIF0 Mouse Protein arginine N-methyltransferase 1 More
基因名称	PRMT1
全名	protein arginine methyltransferase 1
背景介绍	This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
生物功能	Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. [UniProt]
细胞定位	Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytosol. Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity). [UniProt]
预测分子量	42 kDa

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