概述

产品描述	Rabbit Polyclonal antibody recognizes PRKRA
反应物种	Hu, Ms, Rat
应用	IHC-P, WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	PRKRA
抗原物种	Human
抗原	Synthetic peptide of Human PRKRA.
偶联标记	Un-conjugated
別名	PACT; DYT16; Protein kinase, interferon-inducible double-stranded RNA-dependent activator; HSD14; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; PKR-associated protein X; RAX

应用说明

应用建议	应用 推荐稀释比 IHC-P 1:20 - 1:50 WB 1:1000
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
检测时间	O75569

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
浓度	Batch dependent
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 23992 Mouse PRKRA GeneID: 311130 Rat PRKRA GeneID: 8575 Human PRKRA Swiss-port # O75569 Human Interferon-inducible double-stranded RNA-dependent protein kinase activato Swiss-port # Q4V8C7 Rat Interferon-inducible double-stranded RNA-dependent protein kinase activator Swiss-port # Q9WTX2 Mouse Interferon-inducible double-stranded RNA-dependent protein kinase activato More
基因名称	PRKRA
全名	protein kinase, interferon-inducible double stranded RNA dependent activator
背景介绍	This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]
生物功能	Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity). [UniProt]
细胞定位	Cytoplasm, perinuclear region. Cytoplasm. [UniProt]
预测分子量	34 kDa
翻译后修饰	Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress. [UniProt]

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