ARG43955

anti-PRKCSH antibody

anti-PRKCSH antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes PRKCSH
反应物种 Hu, Ms, Rat
应用 ELISA, FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PRKCSH
抗原物种 Human
抗原 Human PRKCSH recombinant protein
表达系统 E.coli
偶联标记 Un-conjugated
Protein Full name Glucosidase 2 subunit beta
別名 PRKCSH; Protein Kinase C Substrate 80K-H; Glucosidase 2 Subunit Beta; VASAP-60; G19P1; GIIB; Protein Kinase C Substrate 60.1 KDa Protein Heavy Chain; Advanced Glycation End-Product Receptor 2; Glucosidase II Subunit Beta; Hepatocystin; PKCSH; PCLD; PLD1; Protein Kinase C Substrate, 80 Kda Protein; Glucosidase II Beta Subunit; Polycystic Liver Disease; AGE-Binding Receptor 2; 80K-H Protein; AGE-R2; PCLD1

应用说明

应用建议
应用 推荐稀释比
ELISA0.1-0.5 µg/ml
FACS1-3 µg /1x10^6 cells
ICC/IF5 µg/ml
WB0.1-0.25 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified with Immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 19089 Mouse PRKCSH

GeneID: 5589 Human PRKCSH

Swiss-port # O08795 Mouse Glucosidase 2 subunit beta

Swiss-port # P14314 Human Glucosidase 2 subunit beta

基因名称 PRKCSH
全名 Protein Kinase C Substrate 80K-H
背景介绍 This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.
生物功能 Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature glycoproteins.
细胞定位 Endoplasmic reticulum
预测分子量 59 kDa
翻译后修饰 Disulfide bond, Glycoprotein, Phosphoprotein

检测图片 (5) Click the Picture to Zoom In

  • ARG43955 anti-PRKCSH antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43955 anti-PRKCSH antibody at 5 μg/ml dilution.

  • ARG43955 anti-PRKCSH antibody WB image

    Western blot: 293T and Hela stained with ARG43955 anti-PRKCSH antibody at 0.5 μg/mL dilution.

  • ARG43955 anti-PRKCSH antibody FACS image

    Flow Cytometry: SiHa cells stained with ARG43955 anti-PRKCSH antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43955 anti-PRKCSH antibody WB image

    Western blot: Rat liver and L6 stained with ARG43955 anti-PRKCSH antibody at 0.5 μg/ml dilution.

  • ARG43955 anti-PRKCSH antibody WB image

    Western blot: mouse liver and C2C12 stained with ARG43955 anti-PRKCSH antibody at 0.5 μg/ml dilution.