ARG65048
anti-PRDM1 / Blimp 1 antibody
anti-PRDM1 / Blimp 1 antibody for Flow cytometry,ICC/IF,Western blot and Human
Gene Regulation antibody; Immune System antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PRDM1 / Blimp 1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Dog, Pig |
| 应用 | FACS, ICC/IF, WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PRDM1 / Blimp 1 |
| 抗原物种 | Human |
| 抗原 | C-DISDNADRLEDVED |
| 偶联标记 | Un-conjugated |
| 別名 | Beta-interferon gene positive regulatory domain I-binding factor; PR domain zinc finger protein 1; BLIMP-1; BLIMP1; PRDI-binding factor 1; PR domain-containing protein 1; EC 2.1.1.-; PRDI-BF1; Positive regulatory domain I-binding factor 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRDM1 |
| 全名 | PR/SET domain 1 |
| 背景介绍 | This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008] |
| 生物功能 | Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (By similarity). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (PubMed:1851123). Drives the maturation of B-lymphocytes into Ig secreting cells (PubMed:12626569). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (By similarity). [UniProt] |
| 研究领域 | Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 92 kDa |
| 翻译后修饰 | Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. |
检测图片 (3) Click the Picture to Zoom In
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ARG65048 anti-PRDM1 / Blimp 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG65048 anti-PRDM1 / Blimp 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65048 anti-PRDM1 / Blimp 1 antibody WB image
Western blot: 35 µg of A431 (A), K562 (B) and K562 nucleus (C) lysates (in RIPA buffer) stained with ARG65048 anti-PRDM1 / Blimp 1 antibody at 0.1 µg/ml (A), 2 µg/ml (B) and 1 µg/ml (C) dilutions and incubated at RT for 1 hour.
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ARG65048 anti-PRDM1 / Blimp 1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A431 cells permeabilized with 0.5% Triton. Cells were stained with ARG65048 anti-PRDM1 / Blimp 1 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
