ARG63788

anti-PPY / Pancreatic Polypeptide antibody

anti-PPY / Pancreatic Polypeptide antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes PPY / Pancreatic Polypeptide
反应物种 Hu, Rat
预测物种 Ms, Dog
应用 FACS, ICC/IF, IHC-P, WB
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 PPY / Pancreatic Polypeptide
抗原物种 Human
抗原 C-TRPRYGKRHKEDT
偶联标记 Un-conjugated
別名 PP; Obinepitide; Pancreatic polypeptide; PNP; Pancreatic prohormone; PI; PH

应用说明

应用建议
应用 推荐稀释比
FACS10 µg/ml
ICC/IFAssay - dependent
IHC-P3 - 5 µg/ml
WBAssay - dependent
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) or microwave tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 24677 Rat PPY

GeneID: 5539 Human PPY

Swiss-port # P01298 Human Pancreatic prohormone

Swiss-port # P06303 Rat Pancreatic prohormone

背景介绍 This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. [provided by RefSeq, Jul 2008]
研究领域 Signaling Transduction antibody
预测分子量 10 kDa

检测图片 (4) Click the Picture to Zoom In

  • ARG63788 anti-PPY / Pancreatic Polypeptide antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63788 anti-PPY / Pancreatic Polypeptide antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63788 anti-PPY / Pancreatic Polypeptide antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed U2OS cells permeabilized with 0.5% Triton. Cells were stained with ARG63788 anti-PPY / Pancreatic Polypeptide antibody (blue line) at 10 µg/ml for 1 hour, followed by incubation with Alexa Fluor® 488 labelled secondary antibody. IgG control: Unimmunized Goat IgG (black line), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.

  • ARG63788 anti-PPY / Pancreatic Polypeptide antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63788 anti-PPY / Pancreatic Polypeptide antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63788 anti-PPY / Pancreatic Polypeptide antibody IHC-P image

    Immunohistochemistry: Paraffin embedded Human pancreas tissue. Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63788 anti-PPY / Pancreatic Polypeptide antibody at 3 µg/ml dilution followed by HRP-staining.