ARG43929

anti-PPP3CA antibody

anti-PPP3CA antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Rat

概述

产品描述 Rabbit Polyclonal antibody recognizes PPP3CA
反应物种 Hu, Rat
应用 ELISA, FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PPP3CA
抗原物种 Human
抗原 Human PPP3CA recombinant protein
表达系统 E.coli
偶联标记 Un-conjugated
Protein Full name Protein phosphatase 3 catalytic subunit alpha
別名 PPP3CA; Protein Phosphatase 3 Catalytic Subunit Alpha; Calcineurin A Alpha; PPP2B; CALNA; CNA1; CAM-PRP Catalytic Subunit; EC 3.1.3.16; CNA Alpha; CALN; Protein Phosphatase 3 (Formerly 2B), Catalytic Subunit, Alpha Isoform (Calcineurin A Alpha); Serine/Threonine-Protein Phosphatase 2B Catalytic Subunit Alpha Isoform; Protein Phosphatase 3 (Formerly 2B), Catalytic Subunit, Alpha Isoform; Protein Phosphatase 2B, Catalytic Subunit, Alpha Isoform; Calmodulin-Dependent Calcineurin A Subunit Alpha Isoform; Protein Phosphatase 3, Catalytic Subunit, Alpha Isozyme; Protein Phosphatase 3 Catalytic Subunit Alpha Isozyme; ACCIID; CALNA1; IECEE1; DEE91; IECEE; CCN1; CNA

应用说明

应用建议
应用 推荐稀释比
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified with Immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 24674 Rat PPP3CA

GeneID: 5530 Human PPP3CA

Swiss-port # P63329 Rat Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Swiss-port # Q08209 Human Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

基因名称 PPP3CA
全名 Protein Phosphatase 3 Catalytic Subunit Alpha
背景介绍 Enables several functions, including ATPase binding activity; calmodulin binding activity; and calmodulin-dependent protein phosphatase activity. Involved in several processes, including calcineurin-NFAT signaling cascade; peptidyl-serine dephosphorylation; and response to calcium ion. Located in several cellular components, including cytosol; dendritic spine; and nucleoplasm. Part of calcineurin complex. Colocalizes with cytoplasmic side of plasma membrane. Implicated in developmental and epileptic encephalopathy 91. Biomarker of focal segmental glomerulosclerosis and schizophrenia.
生物功能 Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca2+-mediated signals.
细胞定位 Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse
预测分子量 59 kDa
翻译后修饰 Acetylation, Nitration, Phosphoprotein

检测图片 (4) Click the Picture to Zoom In

  • ARG43929 anti-PPP3CA antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG43929 anti-PPP3CA antibody at 5 μg/ml dilution.

  • ARG43929 anti-PPP3CA antibody WB image

    Western blot: HeLa and U-87MG stained with ARG43929 anti-PPP3CA antibody at 0.5 μg/mL dilution.

  • ARG43929 anti-PPP3CA antibody FACS image

    Flow Cytometry: SiHa cells stained with ARG43929 anti-PPP3CA antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43929 anti-PPP3CA antibody WB image

    Western blot: C6 stained with ARG43929 anti-PPP3CA antibody at 0.5 μg/mL dilution.