ARG40162
anti-PPP2CA / PPP2CB antibody
anti-PPP2CA / PPP2CB antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PPP2CA / PPP2CB |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PPP2CA / PPP2CB |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human PPP2CA + PPP2CB. |
| 偶联标记 | Un-conjugated |
| 別名 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2Ac; EC 3.1.3.16; PP2Calpha; Replication protein C; RP-C; PP2CA; PP2A-alpha |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | T47D | ||||||
| 实际分子量 | 36 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PPP2CA |
| 全名 | protein phosphatase 2, catalytic subunit, alpha isozyme |
| 背景介绍 | This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008] |
| 生物功能 | PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGO2 (By similarity). [UniProt] |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. May be monoubiquitinated by NOSIP. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG40162 anti-PPP2CA / PPP2CB antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon stained with ARG40162 anti-PPP2CA / PPP2CB antibody.
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ARG40162 anti-PPP2CA / PPP2CB antibody WB image
Western blot: T47D cell lysate stained with ARG40162 anti-PPP2CA / PPP2CB antibody.
