ARG57029
anti-PPM1F antibody [6D11]
anti-PPM1F antibody [6D11] for ICC/IF,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [6D11] recognizes PPM1F |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 6D11 |
| 同位型 | IgG1, kappa |
| 靶点名称 | PPM1F |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-454 of Human PPM1F. |
| 偶联标记 | Un-conjugated |
| 別名 | hFEM-2; FEM-2; CAMKP; POPX2; EC 3.1.3.16; Partner of PIX 2; hFem-2; Protein phosphatase 1F; CaM-kinase phosphatase; CaMKPase; Ca; Protein fem-2 homolog; 2+ |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PPM1F |
| 全名 | protein phosphatase, Mg2+/Mn2+ dependent, 1F |
| 背景介绍 | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. The overexpression of this phosphatase or CAMK2G has been shown to mediate caspase-dependent apoptosis. An alternatively spliced transcript variant has been identified, but its full-length nature has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis. [UniProt] |
| 预测分子量 | 50 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG57029 anti-PPM1F antibody [6D11] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG57029 anti-PPM1F antibody [6D11] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57029 anti-PPM1F antibody [6D11] WB image
Western blot: 40 µg of Jurkat cell lysate stained with ARG57029 anti-PPM1F antibody [6D11] at 1:1000.
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ARG57029 anti-PPM1F antibody [6D11] WB image
Western blot: 2 µg of 1) 293T cell lysate, 2) PPM1F Transfected 293T cell lysate stained with ARG57029 anti-PPM1F antibody [6D11] at 1:1000.
