ARG45462

anti-PPIE antibody [7F2]

anti-PPIE antibody [7F2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

概述

产品描述 Mouse Monoclonal antibody [7F2] recognizes PPIE
反应物种 Hu, Ms, Rat
应用 FACS, ICC/IF, IHC-P, WB
宿主 Mouse
克隆 Monoclonal
克隆号 7F2
同位型 IgG2a
靶点名称 PPIE
抗原物种 Human
抗原 Recombinant protein containing to human PPIE.
偶联标记 Un-conjugated
別名 Cyclophilin-33; CYP-33; Cyclophilin E; PPIase E; EC 5.2.1.8; Peptidyl-prolyl cis-trans isomerase E; CYP33; Rotamase E

应用说明

应用建议
应用 推荐稀释比
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 35 kDa

属性

形式 Liquid
纯化 Affinity purified
缓冲液 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 10450 Human PPIE

GeneID: 56031 Mouse PPIE

Swiss-port # Q9QZH3 Mouse Peptidyl-prolyl cis-trans isomerase E

Swiss-port # Q9UNP9 Human Peptidyl-prolyl cis-trans isomerase E

基因名称 PPIE
全名 peptidylprolyl isomerase E (cyclophilin E)
背景介绍 The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010]
生物功能 PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. [UniProt]
细胞定位 Nucleus. [UniProt]
预测分子量 33 kDa
翻译后修饰 Phosphoprotein. [UniProt]

检测图片 (7) Click the Picture to Zoom In

  • ARG45462 anti-PPIE antibody [7F2] IHC-P image

    Immunohistochemistry: Human placenta stained with ARG45462 anti-PPIE antibody [7F2] at 2 μg/ml dilution.

  • ARG45462 anti-PPIE antibody [7F2] ICC/IF image

    Immunofluorescence: Hela stained with ARG45462 anti-PPIE antibody [7F2] at 5 μg/ml dilution.

  • ARG45462 anti-PPIE antibody [7F2] WB image

    Western blot: HEK293 stained with ARG45462 anti-PPIE antibody [7F2] at 0.5 μg/ml dilution.

  • ARG45462 anti-PPIE antibody [7F2] FACS image

    Flow Cytometry: JK stained with ARG45462 anti-PPIE antibody [7F2] at 1 µg/10^6 cells dilution.

  • ARG45462 anti-PPIE antibody [7F2] IHC-P image

    Immunohistochemistry: Rat cardiac stained with ARG45462 anti-PPIE antibody [7F2] at 2 μg/ml dilution.

  • ARG45462 anti-PPIE antibody [7F2] WB image

    Western blot: Rat lung stained with ARG45462 anti-PPIE antibody [7F2] at 0.5 μg/ml dilution.

  • ARG45462 anti-PPIE antibody [7F2] WB image

    Western blot: HEPA1-6 stained with ARG45462 anti-PPIE antibody [7F2] at 0.5 μg/ml dilution.