ARG57082
anti-PPCS antibody [10D11]
anti-PPCS antibody [10D11] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [10D11] recognizes PPCS |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 10D11 |
| 同位型 | IgG1, kappa |
| 靶点名称 | PPCS |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-311 of Human PPCS. |
| 偶联标记 | Un-conjugated |
| 別名 | Phosphopantothenoylcysteine synthetase; PPC synthetase; EC 6.3.2.5; Phosphopantothenate--cysteine ligase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9HAB8 Human Phosphopantothenate--cysteine ligase |
|---|---|
| 基因名称 | PPCS |
| 全名 | phosphopantothenoylcysteine synthetase |
| 背景介绍 | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. [UniProt] |
| 预测分子量 | 34 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG57082 anti-PPCS antibody [10D11] WB image
Western blot: 40 µg of 1) Jurkat cell lysate, 2) HepG2 cell lysate, 3) HeLa cell lysate, 4) A549 cell lysate, 5) MCF7 cell lysate, 6) SK-OV-3 cell lysate stained with ARG57082 anti-PPCS antibody [10D11] at 1:1000.
