ARG55241

anti-PPAR gamma antibody

anti-PPAR gamma antibody for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody
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publication_link 参考文献8

概述

产品描述

Rabbit Polyclonal antibody recognizes PPAR gamma

反应物种 Hu, Ms, Rat
应用 ICC/IF, IHC-Fr, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PPAR gamma
抗原物种 Human
抗原 KLH-conjugated synthetic peptide corresponding to aa. 2-35 (N-terminus) of Human PPAR gamma.
偶联标记 Un-conjugated
別名 PPARgamma; PPAR-gamma; GLM1; PPARG2; PPARG1; CIMT1; NR1C3; Nuclear receptor subfamily 1 group C member 3; Peroxisome proliferator-activated receptor gamma

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WB1:500 - 1:2000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purification with Protein A and immunogen peptide.
缓冲液 PBS and 0.09% (W/V) Sodium azide
抗菌剂 0.09% (W/V) Sodium azide
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 19016 Mouse PPARG

GeneID: 25664 Rat PPARG

GeneID: 5468 Human PPARG

基因名称 PPARG
全名 peroxisome proliferator-activated receptor gamma
背景介绍 This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]
生物功能 Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity). [UniProt]
细胞定位 Nucleus. Cytoplasm. Note=Redistributed from the nucleus to the cytosol through a MAP2K1/MEK1-dependent manner CCRN4L/NOC enhances its nuclear translocation
研究领域 Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody
预测分子量 58 kDa
翻译后修饰 O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.
Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity).

检测图片 (3) Click the Picture to Zoom In

  • ARG55241 anti-PPAR gamma antibody IHC-P image

    Immunohistochemistry: Mouse bone stained with ARG55241 anti-PPAR gamma antibody.

    From Jingyuan Wen et al. Biomed Pharmacother., (2023) doi: 10.1016/j.biopha.2022.114122, Fig. 5C.

  • ARG55241 anti-PPAR gamma antibody WB image

    Western blot: 30 µg of MCF7 and MDA-MB-231 (PPAR gamma low expression cell line) stained with ARG55241 anti-PPAR gamma antibody at 1:1000 dilution.

  • ARG55241 anti-PPAR gamma antibody WB image

    Western blot: 30 µg of Rat liver lysate stained with ARG55241 anti-PPAR gamma antibody at 1:1000 dilution.

客户反馈

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anti-PPAR gamma antibody

Application:WB

Sample:U87-MG (untreated or treated with cannabinoid)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:16 hours

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:HRP

nuts_pic      Excellent

anti-PPAR gamma antibody

Application:WB

Sample:Rat liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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anti-PPAR gamma antibody

Application:WB

Sample:MCF7 and MDA-MB-231 (PPAR gamma low expression cell line)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

文献引用

Chondroprotective Mechanism of Eucommia ulmoides Oliv.-Glycyrrhiza uralensis Fisch. Couplet Medicines in Knee Osteoarthritis via Experimental Study and Network Pharmacology Analysis

IHC-P / Mouse

Pinger Wang et al.
Drug Des Devel Ther.,  (2023)

publication_link

 

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Glycyrrhizic acid alters the hyperoxidative stress-induced differentiation commitment of MSCs by activating the Wnt/β-catenin pathway to prevent SONFH

WB / Human

Huihui Xu et al.
Food Funct.,  (2023)

publication_link

 

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Qiangguyin inhibited fat accumulation in OVX mice through the p38 MAPK signaling pathway to achieve anti-osteoporosis effects

IHC-P / Mouse

Jingyuan Wen et al.
Biomed Pharmacother.,  (2023)

publication_link

 

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Hyperkeratinization and Proinflammatory Cytokine Expression in Meibomian Glands Induced by Staphylococcus aureus

WB, IHC-P / Mouse

Hua Chen et al.
Invest Ophthalmol Vis Sci.,  (2021)

publication_link

 

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Platelet-rich plasma promotes bone formation, restrains adipogenesis and accelerates vascularization to relieve steroids-induced osteonecrosis of the femoral head.

IHC-P / Rat

Hui-Hui Xu et al.
Platelets.,  (2020)

publication_link

 

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Organotypic Culture of Mouse Meibomian Gland: A Novel Model to Study Meibomian Gland Dysfunction In Vitro.

IHC-Fr, WB / Mouse

Xu Kang-Kang et al.
Invest Ophthalmol Vis Sci.,  (2020)

publication_link

 

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Genome-wide microRNA screening reveals miR-582-5p as a mesenchymal stem cell-specific microRNA in subchondral bone of the human knee joint.

WB / Human WB / Mouse

Wang Pinger et al.
J Cell Physiol.,  (2019)

publication_link

 

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Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells.

WB / IF / Rat

Manabe Aya et al.
J Cell Physiol.,  (2018)

publication_link

 

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