ARG64937

anti-PP2A / PPP2R4 antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

100 μg

499

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Goat Polyclonal antibody recognizes PP2A / PPP2R4
反应物种	Hu, Ms, Rat
预测物种	Cow, Dog
应用	IHC-P, WB
特异性	This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.
宿主	Goat
克隆	Polyclonal
同位型	IgG
靶点名称	PP2A / PPP2R4
抗原物种	Human
抗原	C-GSLLPIHPVTSG
偶联标记	Un-conjugated
別名	Serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; PTPA; EC 5.2.1.8; PP2A; Phosphotyrosyl phosphatase activator; PR53; PP2A, subunit B', PR53 isoform; Serine/threonine-protein phosphatase 2A regulatory subunit B'

应用说明

应用建议

应用	推荐稀释比
IHC-P	3 - 5 µg/ml
WB	0.5 - 2 µg/ml

应用说明

WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from goat serum by antigen affinity chromatography.
缓冲液	Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂	0.02% Sodium azide
稳定剂	0.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 110854 Mouse PPP2R4 GeneID: 5524 Human PPP2R4 Swiss-port # P58389 Mouse Serine/threonine-protein phosphatase 2A activator Swiss-port # Q15257 Human Serine/threonine-protein phosphatase 2A activator
背景介绍	Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
研究领域	Signaling Transduction antibody; PP2A alpha/beta antibody; Loading control antibody
预测分子量	41 kDa

检测图片 (2) Click the Picture to Zoom In

ARG64937 anti-PP2A / PPP2R4 antibody WB image

Western blot: Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35 µg protein in RIPA buffer) stained with ARG64937 anti-PP2A / PPP2R4 antibody at 0.5 µg/ml dilution.
ARG64937 anti-PP2A / PPP2R4 antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64937 anti-PP2A / PPP2R4 antibody at 3.75 µg/ml dilution followed by AP-staining.