ARG41571
anti-PON1 / paraoxonase 1 antibody
anti-PON1 / paraoxonase 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PON1 / paraoxonase 1 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PON1 / paraoxonase 1 |
| 抗原物种 | Rat |
| 抗原 | Recombinant protein corresponding to A30-D274 of Rat PON1 / paraoxonase 1. |
| 偶联标记 | Un-conjugated |
| 別名 | K-45; Aromatic esterase 1; MVCD5; EC 3.1.1.81; A-esterase 1; PON; Serum paraoxonase/arylesterase 1; ESA; Serum aryldialkylphosphatase 1; EC 3.1.8.1; EC 3.1.1.2; PON 1 |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| 实际分子量 | ~ 43 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PON1 |
| 全名 | paraoxonase 1 |
| 背景介绍 | The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008] |
| 生物功能 | Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. [UniProt] |
| 细胞定位 | Secreted, extracellular space. [UniProt] |
| 预测分子量 | 40 kDa |
| 翻译后修饰 | Glycosylated. The signal sequence is not cleaved. Present in two forms, form B contains a disulfide bond, form A does not. [UniProt] |
检测图片 (5) Click the Picture to Zoom In
-
ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
-
ARG41571 anti-PON1 / paraoxonase 1 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat liver and Mouse liver lysates stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
-
ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
-
ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
-
ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
