ARG59949
anti-POLR2H / RPB8 antibody
anti-POLR2H / RPB8 antibody for Immunoprecipitation,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes POLR2H / RPB8 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IP, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | POLR2H / RPB8 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-150 of Human POLR2H (NP_006223.2). |
| 偶联标记 | Un-conjugated |
| 別名 | DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; DNA-directed RNA polymerases I, II, and III subunit RPABC3; hRPB8; RPB8; RPB8 homolog; RPB17; RPABC3; DNA-directed RNA polymerase II subunit H; RNA polymerases I, II, and III subunit ABC3 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Mouse thymus and 293T | ||||||
| 实际分子量 | 17 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P52434 Human DNA-directed RNA polymerases I, II, and III subunit RPABC3 Swiss-port # Q923G2 Mouse DNA-directed RNA polymerases I, II, and III subunit RPABC3 |
|---|---|
| 基因名称 | POLR2H |
| 全名 | polymerase (RNA) II (DNA directed) polypeptide H |
| 背景介绍 | The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013] |
| 生物功能 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. [UniProt] |
| 细胞定位 | Nucleus, nucleolus. [UniProt] |
| 预测分子量 | 17 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG59949 anti-POLR2H / RPB8 antibody WB image
Western blot: 25 µg of Mouse thymus and 293T cell lysates stained with ARG59949 anti-POLR2H / RPB8 antibody at 1:1000 dilution.
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ARG59949 anti-POLR2H / RPB8 antibody IP image
Immunoprecipitation: 200 µg extracts of MCF7 cells immunoprecipitated and stained with ARG59949 anti-POLR2H / RPB8 antibody at 1:1000 dilution.
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ARG59949 anti-POLR2H / RPB8 antibody WB image
Western blot: 25 µg of Mouse thymus and 293T cell lysates stained with ARG59949 anti-POLR2H / RPB8 antibody at 1:1000 dilution through one-step method.
