ARG43913

anti-POLR2H / RPB8 antibody

anti-POLR2H / RPB8 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes POLR2H / RPB8
反应物种 Hu
应用 ELISA, FACS, ICC/IF, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 POLR2H / RPB8
抗原物种 Human
抗原 Human POLR2H / RPB8 recombinant protein
表达系统 E.coli
偶联标记 Un-conjugated
Protein Full name DNA-directed RNA polymerases I, II, and III subunit RPABC3
別名 POLR2H; RNA Polymerase II, I And III Subunit H; RPB8; DNA-Directed RNA Polymerases I, II, And III 17.1 KDa Polypeptide; DNA-Directed RNA Polymerases I, II, And III Subunit RPABC3; Polymerase (RNA) II (DNA Directed) Polypeptide H; DNA-Directed RNA Polymerase II Subunit H; RNA Polymerase II Subunit H; RPB8 Homolog; RPB17; RNA Polymerases I, II, And III Subunit ABC3; Polymerase (RNA) II Subunit H; RPABC3; HRPB8

应用说明

应用建议
应用 推荐稀释比
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purified with Immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 5437 Human POLR2H

Swiss-port # P52434 Human DNA-directed RNA polymerases I, II, and III subunit RPABC3

基因名称 POLR2H
全名 RNA Polymerase II, I And III Subunit H
背景介绍 The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.
生物功能 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.
细胞定位 DNA-directed RNA polymerase, Nucleus
预测分子量 17 kDa
翻译后修饰 Acetylation

检测图片 (3) Click the Picture to Zoom In

  • ARG43913 anti-POLR2H / RPB8 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG43913 anti-POLR2H / RPB8 antibody at 5 μg/ml dilution.

  • ARG43913 anti-POLR2H / RPB8 antibody WB image

    Western blot: Hela and MCF-7 stained with ARG43913 anti-POLR2H / RPB8 antibody at 0.5 μg/mL dilution.

  • ARG43913 anti-POLR2H / RPB8 antibody FACS image

    Flow Cytometry: U87 cells stained withARG43913 anti-POLR2H / RPB8 antibody (blue) at 1 μg/1x10^6 cells dilution.