ARG59947
anti-POLR2F antibody
anti-POLR2F antibody for Western blot and Human,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes POLR2F |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | POLR2F |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 1-127 of Human POLR2F (NP_068809.1). |
| 偶联标记 | Un-conjugated |
| 別名 | POLRF; RPB6; RPB6 homolog; HRBP14.4; DNA-directed RNA polymerases I, II, and III subunit RPABC2; RPABC2; RPB14.4; DNA-directed RNA polymerase II subunit F; RPC15; RPABC14.4; RNA polymerases I, II, and III subunit ABC2; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide |
应用说明
| 应用建议 |
|
||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Rat heart | ||||
| 实际分子量 | 14 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O88828 Rat DNA-directed RNA polymerases I, II, and III subunit RPABC2 Swiss-port # P61218 Human DNA-directed RNA polymerases I, II, and III subunit RPABC2 |
|---|---|
| 基因名称 | POLR2F |
| 全名 | polymerase (RNA) II (DNA directed) polypeptide F |
| 背景介绍 | This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| 生物功能 | DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity). [UniProt] |
| 细胞定位 | Nucleus. [UniProt] |
| 预测分子量 | 14 kDa |
检测图片 (1) Click the Picture to Zoom In
-
ARG59947 anti-POLR2F antibody WB image
Western blot: 25 µg of Rat heart lysate stained with ARG59947 anti-POLR2F antibody at 1:3000 dilution.
