ARG56264
anti-POLR2E antibody
anti-POLR2E antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes POLR2E |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | POLR2E |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human POLR2E |
| 偶联标记 | Un-conjugated |
| 別名 | RPB5; RPABC1; DNA-directed RNA polymerases I, II, and III subunit RPABC1; hRPB25; XAP4; RPB5 homolog; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; hsRPB5; RNA polymerases I, II, and III subunit ABC1 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | POLR2E |
| 全名 | polymerase (RNA) II (DNA directed) polypeptide E, 25kDa |
| 背景介绍 | This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq, Jul 2008] |
| 生物功能 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity). [UniProt] |
| 预测分子量 | 25 kDa |
检测图片 (3) Click the Picture to Zoom In
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ARG56264 anti-POLR2E antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG56264 anti-POLR2E antibody.
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ARG56264 anti-POLR2E antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach cancer stained with ARG56264 anti-POLR2E antibody at 1:100 dilution.
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ARG56264 anti-POLR2E antibody WB image
Western blot: HepG2 cell lysate stained with ARG56264 anti-POLR2E antibody.
