ARG57646
anti-POLR2B antibody
anti-POLR2B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes POLR2B |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | POLR2B |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human POLR2B. |
| 偶联标记 | Un-conjugated |
| 別名 | RNA polymerase II subunit 2; RPB2; POL2RB; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II 140 kDa polypeptide; RNA polymerase II subunit B2; DNA-directed RNA polymerase II subunit RPB2; hRPB140; EC 2.7.7.6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 | ||||||
| 实际分子量 | ~ 134 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P30876 Human DNA-directed RNA polymerase II subunit RPB2 Swiss-port # Q8CFI7 Mouse DNA-directed RNA polymerase II subunit RPB2 |
|---|---|
| 基因名称 | POLR2B |
| 全名 | polymerase (RNA) II (DNA directed) polypeptide B, 140kDa |
| 背景介绍 | This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| 生物功能 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity). [UniProt] |
| 细胞定位 | Nucleus [UniProt] |
| 预测分子量 | 134 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG57646 anti-POLR2B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver stained with ARG57646 anti-POLR2B antibody at 1:100 dilution.
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ARG57646 anti-POLR2B antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG57646 anti-POLR2B antibody at 1:1000 dilution.
