ARG43923
anti-POLR1B antibody
anti-POLR1B antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes POLR1B |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | POLR1B |
| 抗原物种 | Human |
| 抗原 | Human POLR1B recombinant protein |
| 表达系统 | E.coli |
| 偶联标记 | Un-conjugated |
| Protein Full name | DNA-directed RNA polymerase I subunit RPA2 |
| 別名 | POLR1B; RNA Polymerase I Subunit B; RPA135; Rpo1-2; RPA2; DNA-Directed RNA Polymerase I 135 KDa Polypeptide; DNA-Directed RNA Polymerase I Subunit RPA2; Polymerase (RNA) I Polypeptide B, 128kDa; RNA Polymerase I Subunit 2; EC 2.7.7.6; FLJ21921; FLJ10816; DNA-Directed RNA Polymerase I 135kDa Polypeptide; Polymerase (RNA) I Subunit B; A135; TCS4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified with Immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9H9Y6 Human DNA-directed RNA polymerase I subunit RPA2 |
|---|---|
| 基因名称 | POLR1B |
| 全名 | RNA Polymerase I Subunit B |
| 背景介绍 | Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2. |
| 生物功能 | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft. |
| 细胞定位 | Chromosome, DNA-directed RNA polymerase, Nucleus |
| 预测分子量 | 128 kDa |
| 翻译后修饰 | Phosphoprotein |
检测图片 (4) Click the Picture to Zoom In
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ARG43923 anti-POLR1B antibody IHC-P image
Immunohistochemistry: Human lymphoma stained with ARG43923 anti-POLR1B antibody at 2 μg/ml dilution.
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ARG43923 anti-POLR1B antibody ICC/IF image
Immunofluorescence: PC-3 cells stained with ARG43923 anti-POLR1B antibody at 5 μg/ml dilution.
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ARG43923 anti-POLR1B antibody WB image
Western blot: K562, HepG2 and 293T stained with ARG43923 anti-POLR1B antibody at 0.5 μg/mL dilution.
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ARG43923 anti-POLR1B antibody FACS FACS image
Flow Cytometry: A431 cells stained with ARG43923 anti-POLR1B antibody (blue) at 1 μg/1x10^6 cells dilution.
