ARG59379
anti-POLI / DNA Polymerase Iota antibody
anti-POLI / DNA Polymerase Iota antibody for Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes POLI / DNA Polymerase Iota |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | POLI / DNA Polymerase Iota |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 50-350 of Human POLI / DNA Polymerase Iota (NP_009126.2). |
| 偶联标记 | Un-conjugated |
| 別名 | RAD30B; Eta2; RAD3OB; DNA polymerase iota; EC 2.7.7.7; RAD30 homolog B |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | Mouse testis | ||||
| 实际分子量 | 85 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | POLI |
| 全名 | polymerase (DNA directed) iota |
| 生物功能 | Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. [UniProt] |
| 细胞定位 | Nucleus. Note=Accumulates at replication forks after DNA damage. [UniProt] |
| 预测分子量 | 83 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG59379 anti-POLI / DNA Polymerase Iota antibody WB image
Western blot: 25 µg of Mouse testis lysate stained with ARG59379 anti-POLI / DNA Polymerase Iota antibody at 1:1000 dilution.
