ARG42120
anti-POFUT2 antibody
anti-POFUT2 antibody for ICC/IF,Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes POFUT2 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | POFUT2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 240-370 of Human POFUT2 (NP_056042.1). |
| 偶联标记 | Un-conjugated |
| 別名 | FUT13; GDP-fucose protein O-fucosyltransferase 2; C21orf80; Peptide-O-fucosyltransferase 2; O-FucT-2; EC 2.4.1.221 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Rat brain | ||||||
| 实际分子量 | ~ 50 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q8VHI3 Mouse GDP-fucose protein O-fucosyltransferase 2 Swiss-port # Q9Y2G5 Human GDP-fucose protein O-fucosyltransferase 2 |
|---|---|
| 基因名称 | POFUT2 |
| 全名 | protein O-fucosyltransferase 2 |
| 背景介绍 | Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13. [UniProt] |
| 细胞定位 | Endoplasmic reticulum. Golgi apparatus. Note=Mainly located in the endoplasmic reticulum. [UniProt] |
| 预测分子量 | 50 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG42120 anti-POFUT2 antibody WB image
Western blot: 25 µg of Rat brain lysate stained with ARG42120 anti-POFUT2 antibody at 1:1000 dilution.
