ARG62617
anti-PODXL antibody [PN-15]
anti-PODXL antibody [PN-15] for Immunohistochemistry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [PN-15] recognizes PODXL |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | IHC, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | PN-15 |
| 同位型 | IgG1, kappa |
| 靶点名称 | PODXL |
| 抗原物种 | Human |
| 抗原 | Microsomal fraction of human renal cortical tissue homogenate. |
| 偶联标记 | Un-conjugated |
| 別名 | Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1 |
应用说明
| 应用说明 | WB: 1/100 - 1/2000 IHC: 1/10 - 1/500 IHC-P: 1/10 - 1/500 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PODXL |
| 全名 | podocalyxin-like |
| 背景介绍 | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. [UniProt] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody |
| 预测分子量 | 59 kDa |
| 翻译后修饰 | N- and O-linked glycosylated. Sialoglycoprotein (By similarity). |
克隆号文献
The utility of PAX-2 in distinguishing metastatic clear cell renal cell carcinoma from its morphologic mimics: an immunohistochemical study with comparison to renal cell carcinoma marker.
IHC / Human
Expression of renal cell carcinoma-associated markers erythropoietin, CD10, and renal cell carcinoma marker in diffuse malignant mesothelioma and metastatic renal cell carcinoma.
IHC / Human
Renal cell carcinoma to the pancreas in surgical pathology material.
IHC / Human
