ARG56071
anti-PODXL antibody [4F10]
anti-PODXL antibody [4F10] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat
概述
| 产品描述 | Mouse Monoclonal antibody [4F10] recognizes PODXL |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | FACS, ICC/IF, IHC-P |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 4F10 |
| 同位型 | IgM |
| 靶点名称 | PODXL |
| 抗原物种 | Human |
| 抗原 | Recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of Human PODXL. |
| 偶联标记 | Un-conjugated |
| 別名 | Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | PEG precipitation |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PODXL |
| 全名 | podocalyxin-like |
| 背景介绍 | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. [UniProt] |
| 细胞定位 | Cell surface and cytoplasmic |
| 预测分子量 | 59 kDa |
| 翻译后修饰 | N- and O-linked glycosylated. Sialoglycoprotein (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG56071 anti-PODXL antibody [4F10] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human angiosarcoma stained with ARG56071 anti-PODXL antibody [4F10].
