ARG64154

anti-PNPLA2 / ATGL antibody

anti-PNPLA2 / ATGL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

概述

产品描述 Goat Polyclonal antibody recognizes PNPLA2 / ATGL
反应物种 Hu
预测物种 Ms, Rat, Cow, Dog, Pig
应用 IHC-P, WB
特异性 Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 PNPLA2 / ATGL
抗原物种 Human
抗原 C-NIIEVSKEARKR
偶联标记 Un-conjugated
別名 Transport-secretion protein 2; Pigment epithelium-derived factor; TTS2.2; FP17548; 1110001C14Rik; TTS2; PEDF-R; IPLA2-zeta; ATGL; Calcium-independent phospholipase A2; TTS-2.2; Patatin-like phospholipase domain-containing protein 2; iPLA2zeta; Desnutrin; Adipose triglyceride lipase; EC 3.1.1.3

应用说明

应用建议
应用 推荐稀释比
IHC-P2 - 3 µg/ml
WB0.5 - 2 µg/ml
应用说明 IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Purified from goat serum by antigen affinity chromatography.
缓冲液 Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂 0.02% Sodium azide
稳定剂 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 57104 Human PNPLA2

Swiss-port # Q96AD5 Human Patatin-like phospholipase domain-containing protein 2

背景介绍 This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]
研究领域 Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量 55 kDa
翻译后修饰 Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity (By similarity). Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation.

检测图片 (2) Click the Picture to Zoom In

  • ARG64154 anti-PNPLA2 / ATGL antibody WB image

    Western blot: Human Adipose lysate (35 µg protein in RIPA buffer) stained with ARG64154 anti-PNPLA2 / ATGL antibody at 0.5 µg/ml dilution.

  • ARG64154 anti-PNPLA2 / ATGL antibody IHC-P image

    Immunohistochemistry: Paraffin embedded Human Heart. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64154 anti-PNPLA2 / ATGL antibody at 2.5 µg/ml dilution followed by AP-staining.