ARG65299
anti-PLN / Phospholamban antibody
anti-PLN / Phospholamban antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PLN / Phospholamban |
|---|---|
| 反应物种 | Hu, Rat |
| 预测物种 | Cow, Pig |
| 应用 | IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PLN / Phospholamban |
| 抗原物种 | Human |
| 抗原 | KVQYLTRSAIRR-C |
| 偶联标记 | Un-conjugated |
| 別名 | CMH18; PLB; CMD1P; Cardiac phospholamban |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PLN |
| 全名 | phospholamban |
| 背景介绍 | The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008] |
| 生物功能 | Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 6 kDa |
| 翻译后修饰 | Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. |
检测图片 (2) Click the Picture to Zoom In
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ARG65299 anti-PLN / Phospholamban antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65299 anti-PLN / Phospholamban antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65299 anti-PLN / Phospholamban antibody WB image
Western blot: 35 µg of Human heart lysate (protein in RIPA buffer) stained with ARG65299 anti-PLN / Phospholamban antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
