ARG40139
anti-PLK4 antibody
anti-PLK4 antibody for Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PLK4 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PLK4 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 724-758 of Human PLK4. |
| 偶联标记 | Un-conjugated |
| 別名 | SAK; PLK-4; STK18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase Sak; EC 2.7.11.21; Serine/threonine-protein kinase 18; MCCRP2; Polo-like kinase 4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | T47D |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00444 Human Serine/threonine-protein kinase PLK4 |
|---|---|
| 基因名称 | PLK4 |
| 全名 | polo-like kinase 4 |
| 背景介绍 | This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| 生物功能 | Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2. [UniProt] |
| 细胞定位 | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Nucleus, nucleolus. Cleavage furrow. Note=Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows. [UniProt] |
| 预测分子量 | 109 kDa |
| 翻译后修饰 | Ubiquitinated; leading to its degradation by the proteasome. Tyrosine-phosphorylated by TEC. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40139 anti-PLK4 antibody WB image
Western blot: 20 µg of T47D whole cell lysate stained with ARG40139 anti-PLK4 antibody at 1:2000 dilution.
