ARG43614

anti-PLAP / Placental alkaline phosphatase antibody

anti-PLAP / Placental alkaline phosphatase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

概述

产品描述 Rabbit Polyclonal antibody recognizes PLAP / Placental alkaline phosphatase
反应物种 Hu
应用 FACS, ICC/IF, IHC-P, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PLAP / Placental alkaline phosphatase
抗原物种 Human
抗原 Synthetic peptide corresponding to a.a. 403-433 of Human PLAP / Placental alkaline phosphatase.
偶联标记 Un-conjugated
Protein Full name Alkaline phosphatase, placental type
別名 PALP; PLAP; EC 3.1.3.1; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; Alkaline phosphatase, placental type; ALP

应用说明

应用建议
应用 推荐稀释比
FACS1:500 - 1:200 / 1X10^6 cells
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:250
WB1:500 - 1:2500
应用说明 IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量 ~ 70 kDa

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂 0.05% Sodium azide
稳定剂 4% Trehalose
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 250 Human ALPP

Swiss-port # P05187 Human Alkaline phosphatase, placental type

基因名称 ALPP
全名 alkaline phosphatase, placental
背景介绍 The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid Polyesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
生物功能 Alkaline phosphatase that can hydrolyze various phosphate compounds. [UniProt]
细胞定位 Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
预测分子量 58 kDa
翻译后修饰 Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein

检测图片 (5) Click the Picture to Zoom In

  • ARG43614 anti-PLAP / Placental alkaline phosphatase antibody IHC-P image

    Immunohistochemistry: Human placenta ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.

  • ARG43614 anti-PLAP / Placental alkaline phosphatase antibody ICC/IF image

    Immunofluorescence: HeLa cell stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.

  • ARG43614 anti-PLAP / Placental alkaline phosphatase antibody WB image

    Western blot: Human placenta cell lysates stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:1000 dilution.

  • ARG43614 anti-PLAP / Placental alkaline phosphatase antibody FACS image

    Flow Cytometry: SiHa cells stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:500 / 1X10^6 cells.

  • ARG43614 anti-PLAP / Placental alkaline phosphatase antibody ICC/IF image

    Immunofluorescence:Human placenta stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.