ARG65251

anti-PLA2G4A antibody

Datasheet

参考文献1

订购信息

包装

价格 (USD)

100 μg

499

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Goat Polyclonal antibody recognizes PLA2G4A
反应物种	Hu, Ms
预测物种	Rat
应用	FACS, ICC/IF, IHC-P, WB
宿主	Goat
克隆	Polyclonal
同位型	IgG
靶点名称	PLA2G4A
抗原物种	Human
抗原	C-QEKTFRQQRKEHIR
偶联标记	Un-conjugated
別名	Phospholipase A2 group IVA; cPLA2; cPLA2-alpha; Phosphatidylcholine 2-acylhydrolase; PLA2G4; Cytosolic phospholipase A2; EC 3.1.1.5; EC 3.1.1.4

应用说明

应用建议

应用	推荐稀释比
FACS	10 µg/ml
ICC/IF	10 µg/ml
IHC-P	3 - 5 µg/ml
WB	0.3 - 1 µg/ml

应用说明

WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purified from goat serum by antigen affinity chromatography.
缓冲液	Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
抗菌剂	0.02% Sodium azide
稳定剂	0.5% BSA
浓度	0.5 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 18783 Mouse PLA2G4A GeneID: 5321 Human PLA2G4A Swiss-port # P47712 Human Cytosolic phospholipase A2 Swiss-port # P47713 Mouse Cytosolic phospholipase A2
背景介绍	This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008]
研究领域	Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量	85 kDa
翻译后修饰	Activated by phosphorylation at both Ser-505 and Ser-727.

检测图片 (5) Click the Picture to Zoom In

ARG65251 anti-PLA2G4A antibody WB image

Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG65251 anti-PLA2G4A antibody at 1 µg/ml dilution.
ARG65251 anti-PLA2G4A antibody ICC/IF image

Immunofluorescence: Paraformaldehyde fixed A549 cells permeabilized with 0.15% Triton. Cells were stained with ARG65251 anti-PLA2G4A antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG65251 anti-PLA2G4A antibody IHC-P image

Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65251 anti-PLA2G4A antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG65251 anti-PLA2G4A antibody WB image

Western blot: 35 µg of A549 cell lysate (in RIPA buffer) stained with ARG65251 anti-PLA2G4A antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
ARG65251 anti-PLA2G4A antibody FACS image

Flow Cytometry: Paraformaldehyde-fixed A549 cells permeabilized with 0.5% Triton. Cells were stained with ARG65251 anti-PLA2G4A antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).