ARG51336

Name: anti-PKM1 / 2 antibody
Brand: arigo Biolaboratories
SKU: ARG51336
Price: 399 USD
Availability: InStock
Rating: 5.0 (2 reviews)

anti-PKM1 / 2 antibody

Datasheet

订购信息

包装

价格 (USD)

100 μl

399

50 μl

279

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

概述

产品描述	Rabbit Polyclonal antibody recognizes PKM1 / 2
反应物种	Hu, Ms, Rat
应用	WB
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	PKM1 / 2
抗原物种	Human
抗原	Peptide sequence around aa. 36~40(D-S-P-P-I) derived from Human PKM1/2.
偶联标记	Un-conjugated
別名	PK3; PKM2; OIP3; Pyruvate kinase muscle isozyme; CTHBP; HEL-S-30; THBP1; OIP-3; Pyruvate kinase 2/3; Tumor M2-PK; Cytosolic thyroid hormone-binding protein; EC 2.7.1.40; Opa-interacting protein 3; p58; TCB; Pyruvate kinase PKM; Thyroid hormone-binding protein 1

应用说明

应用建议

应用	推荐稀释比
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
缓冲液	PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 18746 Mouse PKM GeneID: 25630 Rat PKM GeneID: 5315 Human PKM Swiss-port # P11980 Rat Pyruvate kinase PKM Swiss-port # P14618 Human Pyruvate kinase PKM Swiss-port # P52480 Mouse Pyruvate kinase PKM More
基因名称	PKM
全名	pyruvate kinase, muscle
背景介绍	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
生物功能	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]
研究领域	Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody
预测分子量	58 kDa
翻译后修饰	ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.

检测图片 (2) Click the Picture to Zoom In

ARG51336 anti-PKM1/2 antibody WB image

Western blot: 20 µg of A549 cell lysates stained with ARG51336 anti-PKM1/2 antibody at 1:500 dilution.
ARG51336 anti-PKM1/2 antibody WB image

Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG51336 anti-PKM1/2 antibody at 1:500 dilution.