ARG40570
anti-PIWIL2 antibody
anti-PIWIL2 antibody for ICC/IF,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PIWIL2 |
|---|---|
| 反应物种 | Hu |
| 应用 | ICC/IF, WB |
| 特异性 | At least three isoforms of PIWIL2 are known to exist; this antibody will only detect the two longest isoforms. The antibody is predicted to not cross-react with other PIWI protein family members. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PIWIL2 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around 17 aa (Center) of Human PIWI-L2. |
| 偶联标记 | Un-conjugated |
| 別名 | PIWIL1L; Piwi-like protein 2; HILI; CT80; mili; Cancer/testis antigen 80 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PIWIL2 |
| 全名 | piwi-like RNA-mediated gene silencing 2 |
| 背景介绍 | PIWIL2 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. Note=Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. [UniProt] |
| 预测分子量 | 110 kDa |
| 翻译后修饰 | Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG40570 anti-PIWIL2 antibody ICC/IF image
Immunofluorescence: HepG2 cells stained with ARG40570 anti-PIWIL2 antibody at 5 µg/ml dilution.
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ARG40570 anti-PIWIL2 antibody WB image
Western blot: HepG2 cell lysate stained with ARG40570 anti-PIWIL2 antibody at 1 µg/ml dilution.
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ARG40570 anti-PIWIL2 antibody ICC/IF image
Immunocytochemistry: HepG2 cells stained with ARG40570 anti-PIWIL2 antibody at 2.5 µg/ml dilution.
