ARG55596
anti-PIK3C2B phospho (Tyr228) antibody
anti-PIK3C2B phospho (Tyr228) antibody for Western blot and Human
Immune System antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PIK3C2B phospho (Tyr228) |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PIK3C2B |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phosphospecific peptide corresponding to aa. 215-250 of Human PIK3C2B. (Phosphorylated at Tyr228) |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.7.1.154; PtdIns-3-kinase C2 subunit beta; PI3K-C2-beta; C2-PI3K; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta; Phosphoinositide 3-kinase-C2-beta |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | A431 + EGF |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # O00750 Human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit bet |
|---|---|
| 基因名称 | PIK3C2B |
| 全名 | phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta |
| 背景介绍 | The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner. [provided by RefSeq, Jul 2008] |
| 生物功能 | Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades. [UniProt] |
| 细胞定位 | Microsome. Cell membrane. Cytoplasm, cytosol. Nucleus. Endoplasmic reticulum. Note=Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 185 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG55596 anti-PIK3C2B phospho (Tyr228) antibody WB image
Western blot: 35 µg of A431 cells untreated or treated with EGF. The blots were stained with ARG55596 anti-PIK3C2B phospho (Tyr228) antibody at 1:1000 dilution.
