ARG45943
anti-PIF1 antibody
anti-PIF1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PIF1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PIF1 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein containing to human PIF1. |
| 偶联标记 | Un-conjugated |
| 別名 | PIF1 5'-To-3' DNA Helicase; PIF1; C15orf20; ATP-dependent DNA helicase PIF1; EC 3.6.4.12; DNA repair and recombination helicase PIF1; PIF1/RRM3 DNA helicase-like protein |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 实际分子量 | 77 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity chromatography purified |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -22°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PIF1 |
| 全名 | PIF1 5'-To-3' DNA Helicase |
| 背景介绍 | This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013] |
| 生物功能 | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. [UniProt] |
| 细胞定位 | Nucleus; Mitochondrion. [UniProt] |
| 预测分子量 | 77 kDa |
| 翻译后修饰 | Phosphoprotein. [UniProt]. [UniProt] |
检测图片 (3) Click the Picture to Zoom In
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ARG45943 anti-PIF1 antibody IHC-P image
Immunohistochemistry: Human renal cancer stained with ARG45943 anti-PIF1 antibody at 2 μg/ml dilution.
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ARG45943 anti-PIF1 antibody WB image
Western blot: A431 stained with ARG45943 anti-PIF1 antibody at 0.5 μg/ml dilution.
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ARG45943 anti-PIF1 antibody FACS image
Flow Cytometry: Hela stained with ARG45943 anti-PIF1 antibody at 1 µg/10^6 cells dilution.
