ARG59326
anti-PIAS4 antibody
anti-PIAS4 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PIAS4 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov, Dog, Hrs, Mk |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PIAS4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to aa. 130-174 of Human PIAS4. (EVRLVKLPFFNMLDELLKPTELVPQNNEKLQESPCIFALTPRQVE) |
| 偶联标记 | Un-conjugated |
| 別名 | PIASy; Piasg; EC 6.3.2.-; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; E3 SUMO-protein ligase PIAS4; PIAS-gamma; PIASY; ZMIZ6 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PIAS4 |
| 全名 | protein inhibitor of activated STAT, 4 |
| 生物功能 | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt] |
| 细胞定位 | Nucleus, PML body. Note=Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies. [UniProt] |
| 预测分子量 | 57 kDa |
| 翻译后修饰 | Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG59326 anti-PIAS4 antibody WB image
Western blot: Rat testis, Mouse testis and HeLa whole cell lysates stained with ARG59326 anti-PIAS4 antibody at 0.5 ug/mL dilution.
