概述

产品描述	Rabbit Polyclonal antibody recognizes PIAS1
反应物种	Hu
应用	ICC/IF
宿主	Rabbit
克隆	Polyclonal
同位型	IgG
靶点名称	PIAS1
抗原物种	Human
抗原	Recombinant fusion protein corresponding to aa. 422-651 of Human PIAS1 (NP_057250.1).
偶联标记	Un-conjugated
別名	Protein inhibitor of activated STAT protein 1; EC 6.3.2.-; Gu-binding protein; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; DDXBP1; GBP; RNA helicase II-binding protein; ZMIZ3; GU/RH-II

应用说明

应用建议	应用 推荐稀释比 ICC/IF 1:50 - 1:200
应用说明	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purified.
缓冲液	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	50% Glycerol
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 8554 Human PIAS1 Swiss-port # O75925 Human E3 SUMO-protein ligase PIAS1
基因名称	PIAS1
全名	protein inhibitor of activated STAT, 1
背景介绍	This gene encodes a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis. [provided by RefSeq, Jul 2008]
生物功能	Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). [UniProt]
细胞定位	Nucleus speckle. Nucleus, PML body. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton. [UniProt]
预测分子量	72 kDa
翻译后修饰	Sumoylated. Dimethylated by PRMT1 at Arg-303 in the late phase of interferon gamma (IFN-gamma) signaling, leading to preferential interaction with STAT1 and thus resulting in release of STAT1 from its target gene. [UniProt]

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