ARG40806
anti-PHKB antibody
anti-PHKB antibody for Western blot and Human,Mouse
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PHKB |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PHKB |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 854-1093 of Human PHKB (NP_000284.1). |
| 偶联标记 | Un-conjugated |
| 別名 | Phosphorylase b kinase regulatory subunit beta; Phosphorylase kinase subunit beta |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | BT-474 | ||||
| 实际分子量 | ~ 120 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q7TSH2 Mouse Phosphorylase b kinase regulatory subunit beta Swiss-port # Q93100 Human Phosphorylase b kinase regulatory subunit beta |
|---|---|
| 基因名称 | PHKB |
| 全名 | phosphorylase kinase, beta |
| 背景介绍 | Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010] |
| 生物功能 | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation. [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor; Cytoplasmic side. [UniProt] |
| 预测分子量 | 125 kDa |
| 翻译后修饰 | Ser-701 is probably phosphorylated by PKA. Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG40806 anti-PHKB antibody WB image
Western blot: 25 µg of BT-474 cell lysate stained with ARG40806 anti-PHKB antibody at 1:1000 dilution.
