ARG44068
anti-PHF1 antibody
anti-PHF1 antibody for Western blot and Human,Mouse,Rat
概述
| 产品描述 | Rabbit Polyclonal recognizes PHF1 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PHF1 |
| 抗原物种 | Human |
| 抗原 | Human PHF1 recombinant protein (Position: L31-H491). |
| 偶联标记 | Un-conjugated |
| 別名 | PHF1; PHD Finger Protein 1; PCL1; TDRD19C; MTF2L2; Tudor Domain Containing 19C; Polycomb-Like Protein 1; Polycomb-Like 1; HPHF1; HPCl1; Testicular Tissue Protein Li 140; Protein PHF1; PHF2 |
应用说明
| 应用建议 |
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| 应用说明 | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PHF1 |
| 全名 | PHD Finger Protein 1 |
| 背景介绍 | This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. |
| 生物功能 | Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. |
| 细胞定位 | Cytoplasm, Cytoskeleton, Nucleus |
| 预测分子量 | 62 kDa |
| 翻译后修饰 | Phosphoprotein |
检测图片 (2) Click the Picture to Zoom In
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ARG44068 anti-PHF1 antibody WB image
Western blot: rat heart stained with ARG44068 anti-PHF1 antibody at 0.5 μg/mL dilution.
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ARG44068 anti-PHF1 antibody WB image
Western blot: mouse hear tand mouse C2C12 stained with ARG44068 anti-PHF1 antibody at 0.5 μg/mL dilution.
