ARG54875
anti-PGK1 antibody
anti-PGK1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PGK1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Mk, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PGK1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 117-145 (Center) of Human PGK1. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 2.7.2.3; Primer recognition protein 2; PGKA; PRP 2; Phosphoglycerate kinase 1; MIG10; Cell migration-inducing gene 10 protein; HEL-S-68p |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PGK1 |
| 全名 | phosphoglycerate kinase 1 |
| 背景介绍 | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014] |
| 生物功能 | In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). [UniProt] |
| 细胞定位 | Cytoplasm. |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 45 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG54875 anti-PGK1 antibody WB image
Western blot: 30 μg of DU145, HepG2, MEF, Mouse ovary and Rat ovary lysates stained with ARG54875 anti-PGK1 antibody at 1:500 dilution.
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ARG54875 anti-PGK1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG54875 anti-PGK1 antibody (green). DAPI (blue) for nuclear staining.
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ARG54875 anti-PGK1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue stained with ARG54875 anti-PGK1 antibody.
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ARG54875 anti-PGK1 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG54875 anti-PGK1 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
