ARG64237
anti-PGC1 alpha antibody
anti-PGC1 alpha antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
概述
| 产品描述 | Goat Polyclonal antibody recognizes PGC1 alpha |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Cow, Dog |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PGC1 alpha |
| 抗原物种 | Human |
| 抗原 | C-DGLFDDSEDESDK |
| 偶联标记 | Un-conjugated |
| 別名 | Ligand effect modulator 6; PGC1; PPARGC-1-alpha; PPARGC1; PGC1A; PPAR-gamma coactivator 1-alpha; PGC-1v; LEM6; PGC-1-alpha; Peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1(alpha) |
应用说明
| 应用建议 |
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| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by antigen affinity chromatography. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PPARGC1A |
| 全名 | peroxisome proliferator-activated receptor gamma, coactivator 1 alpha |
| 背景介绍 | The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 91 kDa |
| 翻译后修饰 | Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2. Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions. Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. |
检测图片 (5) Click the Picture to Zoom In
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ARG64237 anti-PGC1 alpha antibody IHC-P image
Immunohistochemistry: Human Kidney stained with ARG64237 anti-PGC1 alpha antibody at 6 µg/ml dilution.
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ARG64237 anti-PGC1 alpha antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG64237 anti-PGC1 alpha antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64237 anti-PGC1 alpha antibody WB image
Western blot: 35 µg of Rat duodenum lysate (in RIPA buffer) with (B) and without (A) blocking peptide. The blots were stained with ARG64237 anti-PGC1 alpha antibody at 1.0 µg/ml dilution and incubated at RT for 1 hour.
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ARG64237 anti-PGC1 alpha antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG64237 anti-PGC1 alpha antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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ARG64237 anti-PGC1 alpha antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64237 anti-PGC1 alpha antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
客户反馈
Excellent
anti-PGC1 alpha antibody
Application：WB
Sample：1) Mouse kidney, 2) Rat kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
anti-PGC1 alpha antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
