ARG57141
anti-PGAM1 antibody [1G4]
anti-PGAM1 antibody [1G4] for Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody [1G4] recognizes PGAM1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1G4 |
| 同位型 | IgG2a, lambda |
| 靶点名称 | PGAM1 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-254 of Human PGAM1 |
| 偶联标记 | Un-conjugated |
| 別名 | PGAMA; EC 3.1.3.13; Phosphoglycerate mutase isozyme B; HEL-S-35; EC 5.4.2.11; PGAM-B; BPG-dependent PGAM 1; Phosphoglycerate mutase 1; EC 5.4.2.4 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PGAM1 |
| 全名 | phosphoglycerate mutase 1 (brain) |
| 背景介绍 | Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010] |
| 生物功能 | Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. [UniProt] |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme. |
检测图片 (1) Click the Picture to Zoom In
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ARG57141 anti-PGAM1 antibody [1G4] WB image
Western blot: 40 µg of 1) 293T, 2) Jurkat, 3) Raji, 4) A431, and 5) HeLa cell lysates stained with ARG57141 anti-PGAM1 antibody [1G4] at 1:1000.
