ARG66418
anti-PGA5 antibody [SQab18120]
anti-PGA5 antibody [SQab18120] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig
概述
| 产品描述 | Mouse Monoclonal antibody [SQab18120] recognizes PGA5 |
|---|---|
| 反应物种 | Hu, Ms, Pig |
| 应用 | ELISA, FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SQab18120 |
| 同位型 | IgG1, kappa |
| 靶点名称 | PGA5 |
| 抗原物种 | Human |
| 抗原 | Recombinant Human PGA5 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.4.23.1; Pepsin A-5; Pg5; Pepsinogen-5 |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 纯化 | Affinity purification with immunogen. |
|---|---|
| 缓冲液 | PBS (pH 7.4) and 0.01% Thimerosal. |
| 抗菌剂 | 0.01% Thimerosal |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PGA5 |
| 全名 | pepsinogen 5, group I (pepsinogen A) |
| 背景介绍 | This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015] |
| 生物功能 | Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 预测分子量 | 42 kDa |
检测图片 (4) Click the Picture to Zoom In
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ARG66418 anti-PGA5 antibody [SQab18120] ICC/IF image
Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66418 anti-PGA5 antibody [SQab18120] (green) at 1:100 dilution and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66418 anti-PGA5 antibody [SQab18120] FACS image
Flow Cytometry: K562 cells were stained with ARG66418 anti-PGA5 antibody [SQab18120] at 2 µg/ml (red) and without antibody control (black).
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ARG66418 anti-PGA5 antibody [SQab18120] WB image
Western blot: 25 ng of Purified Human pepsinogen I, 25 ng of Purified Human pepsinogen II, 30 µg of Mouse stomach lysate and 10 µl of Human gastric juice stained with ARG66418 anti-PGA5 antibody [SQab18120] at 1:5000 dilution.
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ARG66418 anti-PGA5 antibody [SQab18120] ELISA image
ELISA: The specificity of ARG66418 anti-PGA5 antibody [SQab18120] recognize recombinant proteins. Red: Porcine pepsin; Orange: PGA5; Green: Pepsinogen I; Blue: Pepsinogen II; Black: BSA (negative control).
