ARG42147
anti-PFKL antibody
anti-PFKL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PFKL |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PFKL |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide between aa. 669-699 of Human PFKL. |
| 偶联标记 | Un-conjugated |
| 別名 | PFK-B; 6-phosphofructokinase type B; EC 2.7.1.11; ATP-dependent 6-phosphofructokinase, liver type; PFK-L; Phosphofructo-1-kinase isozyme B; ATP-PFK; Phosphohexokinase |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | HepG2 | ||||||
| 实际分子量 | ~ 85 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17858 Human ATP-dependent 6-phosphofructokinase, liver type |
|---|---|
| 基因名称 | PFKL |
| 全名 | phosphofructokinase, liver |
| 背景介绍 | This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
| 生物功能 | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis (PubMed:22923583). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway (By similarity). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation. [UniProt] |
检测图片 (2) Click the Picture to Zoom In
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ARG42147 anti-PFKL antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma tissue stained with ARG42147 anti-PFKL antibody.
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ARG42147 anti-PFKL antibody WB image
Western blot: HepG2 cell lysate stained with ARG42147 anti-PFKL antibody.
